Were you not able to make the just-completed Human Proteome Organization (HUPO) World Congress in Yokohama, Japan, this year? If not, let LABline tell you about two new products that Thermo Fisher Scientific was showing there: the Exactive Plus EMR and Q Exactive Plus Orbitrap systems, designed to advance intact protein analysis and “quanfirmation.”
The Extended Mass Range (EMR) option for the Thermo Scientific Exactive Plus LC-MS gives proteomics scientists a powerful new tool for challenging high resolution accurate mass (HRAM) intact protein analysis such as investigating the structure, topology, and architecture of targets. These include impurities in monoclonal antibodies, antibody-drug conjugates, PEGylated proteins, oligomerized protein-based drugs, glycoforms, and protein assemblies. The EMR option includes extended m/z range of 350 to 20,000; improved transmission of higher-mass ions for stronger signals; modified HCD pressure and controls for easier optimization of experimental conditions; and access to long transients for improved signal-to-noise ratio.
The mass spectrometry community first learned about “quanfirmation” (the ability to characterize, quantify, and confirm a molecule using one instrument) in 2011 with the debut of the Thermo Scientific Q Exactive hybrid LC-MS. Now, the company is introducing the second generation of this benchtop hybrid HRAM instrument, the Thermo Scientific Q Exactive Plus LC-MS, to further increase performance for applications such as bottom-up proteomics, lipidomics, and high-confidence DMPK qual/quan screening studies. Enhancements include Advanced Quadrupole Technology; data-independent acquisition and parallel reaction monitoring; advanced active beam guide; optional protein mode; and enhanced resolution option. Read about Thermo Fisher’s contributions at HUPO, with tabs to seminars and poster sessions.