Yourgene Health and PacBio announced a collaboration to optimize size selection for long-read sequencing workflows. PacBio has qualified Yourgene’s LightBenchinstrument for size selection of long DNA fragments, enabling high yields for HiFi sequencing data.
Yourgene’s LightBench is an automated electrophoresis instrument, powered by Ranger Technology. This platform uses a patent-protected, machine-vision algorithm to interpret and control gel electrophoresis in real time. It delivers size selection for human genomic DNA and includes the ability to perform fragment length analysis for additional quality control.
PacBio HiFi sequencing is a single-molecule real-time (SMRT) sequencing technology. It produces long (1,000 to 20,000+ bases), reads with uniform genomic coverage and native methylation detection for use in applications such as whole genome reconstruction, haplotype phasing, detection of large and complex variants, and epigenetics.
A new Technical Note was released for PacBio customers titled “Size selection of PacBio SMRTbell libraries with the LightBenchinstrument from Yourgene Health.” The Yourgene LightBench instrument is compatible with the Revio system. The automated LightBench workflow optimizes walk away time, offering a scalable size selection solution for the preparation of PacBio SMRTbell sequencing libraries. Results from the initial study assessing two fragment lengths in 84 human genomic DNA samples from frozen blood for library preparation showed consistent yields for 8 kb or 10 kb size and a zero percent failure rate.