Association for Molecular Pathology publishes best practice guidance for designing and utilizing slice testing approach for diagnostics

Dec. 21, 2023
New expert consensus report authored by representatives from AMP, CAP, and NSGC.

The Association for Molecular Pathology (AMP) published a report that explores specific considerations for a slice testing strategy for diagnostics, including gene selection, analytic performance, coverage, quality, and interpretation.

The report offers expert consensus recommendations and results from an AMP-sponsored survey that compares a slice testing approach with traditional static gene panels and comprehensive exome analysis. The manuscript, “Slice Testing - Considerations from Ordering to Reporting: A Joint Report of the Association for Molecular Pathology, College of American Pathologists, and National Society of Genetic Counselors,” was released online ahead of publication in The Journal of Molecular Diagnostics.

As the number of genes associated with various germline disorders continues to grow, it is becoming more difficult for clinical laboratories to maintain separate assays for interrogating disease focused gene panels. To help overcome this challenge, laboratories are employing a slice testing strategy that utilizes capture backbone to analyze data specific to a set of genes. Similar to traditional disease focused panels, a slice test sequences a broad set of genes but limits the interpretation to a predetermined list of relevant genes. This approach combines the advantages of high-quality gene panels with the flexibility and broad scope of exome sequencing.

The AMP Whole-Exome Sequencing Standards Working Group was established to survey current laboratory practice and develop initial best practice guidance for germline slice testing using exomes. The recommendations were informed by a directed review of existing scientific literature, observational survey data, and the professional experience of the Working Group’s subject matter experts. The report outlines the capabilities, limitations, and considerations when using slice testing for laboratories and clinical providers. These expert consensus recommendations are meant to be a reference guide and not to be interpreted as a restrictive list.

AMP release