In the age of precision medicine, molecular assays will continue to grow in number and importance. Assay developers face complex challenges as they work to find solutions for emerging and unmet needs, and clinical laboratories are often required to quickly implement new IVD tests with short turnaround times. PCR-based molecular diagnostic (MDx) assays have become indispensable in the diagnostic landscape and are widely used in clinical laboratories. The simplicity, high degree of accuracy, and analytical sensitivity of PCR assays make them ideal for diagnostic applications in many settings, including oncology and infectious disease laboratories. Robust PCR technology and chemistry also enable the detection of multiple targets in the same reaction.