Meridian Bioscience has launched its new Air-Dryable Direct RNA/DNA qPCR Blood Mix.
Meridian said it designed the master mix to develop room-temperature stable molecular diagnostic assays that directly amplify RNA from crude whole blood, serum, or plasma. Meridian said the offering helps molecular diagnostic manufacturers develop assays faster.
The ability of qPCR to screen directly from blood samples provides faster and highly sensitive detection of viral infections such as HIV, HBV, or HCV, but also bacterial infections, hereditary diseases, and forensic analyses, Meridian contends.
Blood can also be used as a non-invasive liquid biopsy for examining circulating tumor DNA, RNA, or cells, which is essential in cancer management, allowing prognosis, diagnosis, and monitoring following treatment, the company said. However, PCR inhibitors often found in blood samples can produce false-negative results or reduced limit-of-detection ("LOD") unless costly and time-consuming DNA/RNA purification is used.
Meridian's new Air-Dryable Direct RNA/DNA qPCR Blood Mix can be used on crude samples and eliminates the need for an extraction step.