The new Applied Biosystems TaqMan QSY2 probes from Thermo Fisher Scientific demonstrate improved assay performance retention when transitioning from single-plex to six-plex.
The TaqMan QSY2–quenched probe is a new quencher that enables lower background and greater signal to noise ratio with TaqMan 5’ nuclease qPCR probes. Equipped with the Cyanine 5 and Cyanine 5.5 long wavelength dyes, these probes enable 5th and 6th target multiplexing in the far-red spectrum. In head-to-head studies with their counterparts, TaqMan QSY and QSY2 probes demonstrate greater sensitivity, more dynamic range, and better performance retention when scaling from single-plex to six-plex.
Key applications:
- Microbial pathogen detection in clinical research labs
- Detection of impurities and fluctuations for bioproduction processes in Pharma/Biotech R&D and QC labs
- Gene expression studies in academic research labs