New Applied Biosystems TaqMan QSY2 probes enable multiplexing of research assays

April 2, 2024
Applied Biosystems TaqMan QSY2 probes enable multiplexing of research assays for up to six targets in a single qPCR reaction with TaqMan performance.

The new Applied Biosystems TaqMan QSY2 probes from Thermo Fisher Scientific demonstrate improved assay performance retention when transitioning from single-plex to six-plex.

The TaqMan QSY2–quenched probe is a new quencher that enables lower background and greater signal to noise ratio with TaqMan 5’ nuclease qPCR probes. Equipped with the Cyanine 5 and Cyanine 5.5 long wavelength dyes, these probes enable 5th and 6th target multiplexing in the far-red spectrum. In head-to-head studies with their counterparts, TaqMan QSY and QSY2 probes demonstrate greater sensitivity, more dynamic range, and better performance retention when scaling from single-plex to six-plex.

Key applications:

  • Microbial pathogen detection in clinical research labs
  • Detection of impurities and fluctuations for bioproduction processes in Pharma/Biotech R&D and QC labs  
  • Gene expression studies in academic research labs

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