New England Biolabs (NEB) announced the launch of the NEBNext Low-bias Small RNA Library Prep Kit, designed to minimize biased representation of small RNA species in sequencing data.
"Small RNA species – including rare and non-coding RNAs – have historically been relegated to a black box. Now, researchers are opening that box and realizing these tiny RNAs have an outsized influence on health and disease," said Betsy Young, Ph.D., Senior Product Marketing Manager for the NEBNext portfolio. "However, sequencing bias in preexisting library preparation methods has made it hard for researchers to understand the true diversity and abundance of small RNAs in their samples. This kit was designed to generate RNA libraries with a more accurate representation of small RNA species."
Protocol enhancements include the addition of a novel splint adaptor that increases the diversity of interactions, facilitating ligation and increasing sensitivity, with a streamlined, simplified protocol. As a result, researchers can now confidently analyze all RNA species present in biologically relevant samples. Additional improvements include speed (~3.5 hrs), shelf life (18 months), and input range. Standard and 2´-O-methylated samples can also be processed using the same protocol, with multiplexing enabled through up to 480 compatible UDI primer pairs (available separately).
For more details about the NEBNext Low-bias Small RNA Library Prep Kit, or to request a trial kit, visit https://www.neb.com/en-us/products/e3420-nebnext-low-bias-small-rna-library-prep-kit.
