SeqOnce Biosciences RT-qPCR assay detects SARS-CoV-2 variant

Feb. 16, 2021

SeqOnce Biosciences announced the launch of its new AzureSeq One-Step Universal RT-qPCR kit for the detection of the novel coronavirus SARS-CoV-2 N501Y variant, according to a news release.

The AzureSeq One-Step Universal SARS-CoV-2 RT-qPCR N501Y kit is currently for research use only.

The AzureSeq One-Step Universal SARS-CoV-2 RT-qPCR N501Y kit uses a single-tube reaction targeting the N1 nucleocapsid gene, both alleles of S gene N501Y (A23063T), and includes RNaseP as a control. The assay uses common qPCR instruments with detection channels of FAM, HEX, Cy5, and ROX. The assay does not require viral RNA extraction and has a rapid cycling protocol option.

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