Genomics and proteomics
Automated DNA extraction and PCR setup
The automated CheckExtractor extracts DNA from sample material. The advantages of automated DNA extraction are: The procedure is accurate, reliable, and requires only minimum hands-on time. Up to 288 samples can be processed per day. The CheckExtractor is suitable for different types of sample vessels containing sensitive materials, such as swabs, blood, and urine. A touch screen enables intuitive operation. The sample vessels and reagents are scanned automatically via barcodes, making it possible to closely observe the extraction status of all samples at any time. The individual steps in the analysis process are managed and partly controlled by the laboratory information-management system. In addition, the system enables automated PCR setup: samples are prepared so they only have to be taken from the machine and placed in the thermocycler.
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Greiner Bio-One
Kit for genomic DNA fragmentation
The AIR DNA Fragmentation Kit offers a cost-effective procedure for the fragmentation of genomic DNA in next-generation sequencing sample prep and has been optimized to physically shear uniform fragments of genomic DNA. This fragmentation step is a critical first step for next-generation sequencing of genomic DNA. The kit contains a redesigned nebulizer and optimized buffers, and protocol that results in highly reproducible DNA shearing, with DNA fragment length distribution ideal for next-generation sequencing library preparation for either the Illumina/Solexa or the Roche 454 sequencing platform.
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Bioo Scientific
Increase sensitivity for DNA fragment analysis
The High Sensitivity DNA Kit for quality control, sizing, and quantification of fragmented DNA and DNA libraries in the next-generation-sequencing workflow is used with the 2100 Bioanalyzer. The kit enables users to quantify DNA fragments in the lower single-digit pg/μL concentration range. Many NGS sample-preparation protocols require DNA library amplification by PCR, which can often introduce amplification bias. The high sensitivity significantly lowers the number of required PCR cycles, reducing amplification artifacts and improving the quality of sequencing data. Only 1 μL of material is required per analysis, and the standardized assays and pre-packaged reagent kits are sufficient for 110 samples.
Agilent Technologies
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DNA-extraction instrument
The AutoGenFlex STAR Automated DNA Extraction Instrument features a protocol optimized for this company’s FlexiGene chemistry to isolate highly pure DNA from 40 samples of up to 10 mL each human whole blood and produce 25 µgs to 40 µgs per mL. Featuring a hand-held bar-code scanner for the automated population of sample data, this fully automated, walkaway system has a built-in centrifuge, gripper-type robotic handling, and removable sample- and DNA-storage racks. Providing sample tracking with bar-code identification and reporting software, the instrument can isolate DNA from whole blood, buffy coats, tissues, cells, buccal swabs, Oragene saliva, and more.
Autogen
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